Anti-Aβ antibody treatment promotes the rapid recovery of amyloid-associated neuritic dystrophy in PDAPP transgenic mice
J. Clin. Invest. Robert P. Brendza, et al. 115:428 doi:10.1172/JCI23269 [Go to this article.]

Figure 1
Neuritic plaques and CAA in a living PDAPP;Thy-1:YFP double-transgenic mouse. Using multiphoton microscopy, we were able to observe and monitor amyloid plaques and CAA through cranial windows in PDAPP;Thy-1:YFP double-transgenic mice using the in vivo amyloid-imaging fluorophore methoxy-X04 (blue). These images were taken approximately 24 hours after an i.p. injection of methoxy-X04. Amyloid-associated YFP-labeled dystrophic neurites, as well as unaffected axons, dendrites, and dendritic spines, were visualized by their inherent fluorescence (green). (A–C) A low-magnification view of an area within the cerebral cortex of a PDAPP;Thy-1:YFP double-transgenic mouse, in which prominent neuritic plaques and CAA can be seen. The arrowhead points to CAA and the arrow to a neuritic plaque in the brain parenchyma. (D–F) A higher-magnification view of a neuritic plaque found in the same area shown in A. (A and D) YFP only; (B and E) methoxy-X04–stained amyloid only; (C) merged image of A and B; (F) merged image of D and E. Scale bars: A–C, 200 μm; D–F, 20 μm.