Targeted and restricted complement activation on acrosome-reacted spermatozoa
J. Clin. Invest. Rebecca C. Riley-Vargas, et al. 115:1241 doi:10.1172/JCI23213 [
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Figure 6Confocal microscopy of AR spermatozoa exposed to 10% serum. Spermatozoa were incubated with calcium ionophore to induce the acrosome reaction and then exposed to 10% autologous serum. (
A) The mAb TRA-2-10 labeled with Alexa Fluor 488 recognizes MCP on the IAM. (
B) An mAb to C3d labeled with Alexa Fluor 647 binds to the IAM. (
C) Overlay of
A and
B. C3d deposition is limited to the IAM. Magnification, ×63.
