Intrarenal cells, not bone marrow–derived cells, are the major source for regeneration in postischemic kidney
J. Clin. Invest. Fangming Lin, et al. 115:1756 doi:10.1172/JCI23015 [Go to this article.]

Figure 2
Tubular injury and cell proliferation in EGFP-expressing tubular epithelial cells. PAS staining and immunostaining were performed in the kidney sections of cre^ksp;Z/EG mice with renal IRI. (A) PAS staining of the kidney at 2 days after IRI. Tubular injury is shown by the loss of brush border membrane, cell detachment from the basement membrane, and nuclear condensation in some cells (arrows). (B) Expression of PCNA in tubular cells (red, arrows). (C) Low-power image of BrdU incorporation in renal tubules. Some BrdU-containing cells (red, arrows) colocalized with EGFP-expressing cells (green). The arrowhead indicates BrdU incorporation in an EGFP-negative cell. (D) BrdU incorporation (red, arrow) in the epithelial cells expressing EGFP (green). The nuclei were counterstained with DAPI, and images were merged (B–D). Scale bars: 20 μm.