The cytoskeletal protein ezrin regulates EC proliferation and angiogenesis via TNF-α–induced transcriptional repression of cyclin A
J. Clin. Invest. Raj Kishore, et al. 115:1785 doi:10.1172/JCI22849 [Go to this article.]

Figure 4
DN ezrin attenuates TNF-α effects on cyclin A. (A) Nuclear extracts from serum-treated and TNF-α–treated BAECs, transfected with WT or DN ezrin, were evaluated for CHR-binding activity in EMSAs. Overexpression of DN ezrin significantly blunted TNF-α–induced upregulation of CHR-binding activity. (B) BAECs cotransfected with cyclin A promoter–reporter construct and either WT or DN ezrin constructs and cultured in the presence or absence of TNF-α for 24 hours were analyzed for luciferase activity. The bar graph represents average promoter activity from 3 similar experiments. *P < 0.01. (C) Total RNA from empty vector–, WT ezrin–, or DN ezrin–transfected cells under serum or TNF-α treatment conditions was evaluated for cyclin A mRNA expression by ribonuclease protection assays. A representative autoradiograph from 3 similar experiments is shown.