Neurotrophins promote revascularization by local recruitment of TrkB⁺ endothelial cells and systemic mobilization of hematopoietic progenitors
J. Clin. Invest. Pouneh Kermani, et al. 115:653 doi:10.1172/JCI22655 [Go to this article.]

Figure 7
AdBDNF induces mobilization of hematopoietic cells. (A) Total white blood cells (WBCs) in peripheral blood were quantified at 6 or 11 days after the injection of AdNull (yellow), AdVEGF-A (blue), or AdBDNF (red) into the tail veins of C57BL/6 mice. (B) Flow cytometry analysis of peripheral blood from animals treated with AdBDNF. CD11b⁺ and Sca-1⁺ cells are mobilized by AdBDNF. Percentages indicate percentage of positive cells detected by flow cytometry. (C) Flow cytometry analysis detected a 3.1-fold increase for Sca-1 at day 6 and 3.7 and 3.1-fold increases for Sca-1 and CD11b, respectively, 11 days after injection, in animals treated with AdBDNF. The bar graph illustrates the calculated cell number obtained by multiplying the percentage of positive cells, as measured by flow cytometry, by total WBC count. This experiment was done with 2 animals in each group treated. The numbers above the bars indicate the fold increase in WBC count. (D) Flow cytometry analysis of the peripheral blood in animals injected with AdNull (yellow), AdBDNF (red), or AdBDNF in combination with DC101 antibody (blue), 6 days after injection. Treatment with the DC101 antibody had no effect on the mobilization of Sca-1⁺ cells in animals treated with AdBDNF. The bar graph illustrates the calculated cell number obtained by multiplying the percentage of positive cells, as measured by flow cytometry, by total WBC count. This experiment was performed with 3 animals in each group treated.