Recruitment and expansion of dendritic cells in vivo potentiate the immunogenicity of plasmid DNA vaccines
J. Clin. Invest. Shawn M. Sumida, et al. 114:1334 doi:10.1172/JCI22608 [Go to this article.]

Figure 5
Generalizability of MIP-1α/Flt3L–augmented DNA vaccines. (A) BALB/c mice or C57BL/6 mice were immunized, respectively, with 50 μg HIV env gp120 DNA vaccine or 50 μg SIV gag DNA vaccine, each with or without plasmid MIP-1α and plasmid Flt3L. ELISPOT assays were performed using pooled env peptides and the P18 epitope peptide for the env-vaccinated mice, or pooled gag peptides and the AL11 epitope peptide for the gag-vaccinated mice. (B) ELISPOT assays were performed using splenocytes from env-vaccinated BALB/c mice depleted of CD4⁺ or CD8⁺ T lymphocytes.