Neuronal calcium sensor-1 enhancement of InsP₃ receptor activity is inhibited by therapeutic levels of lithium
J. Clin. Invest. Christina Schlecker, et al. 116:1668 doi:10.1172/JCI22466 [Go to this article.]

Figure 5
PC12 cells overexpressing NCS-1 show increased intracellular calcium release after stimulation by extracellular ATP. (A) Wild-type PC12 cells contain both NCS-1 (green) and InsP₃R1 (red). Scale bar: 50 μm. (B) Representative traces showing calcium release evoked by the addition of 50 μM ATP for 1 minute followed by a washout (dark trace: PC12 cells overexpressing NCS-1; light trace: PC12 cells transfected with empty vector). (C) Representative traces comparing the intracellular calcium release after preincubation with lithium (30 minutes, 10 mM lithium; light trace) with NCS-1 cells that were not treated with lithium (dark trace). Note that lithium did not alter the fluorescence signal but did decrease the NCS-1–dependent increase in calcium release to control levels. (D) Averaged values from at least 3 experiments.