MyD88-dependent induction of allergic Th2 responses to intranasal antigen
J. Clin. Invest. Damani A. Piggott, et al. 115:459 doi:10.1172/JCI22462 [
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Figure 3Phenotype of lung cells in the absence of collagenase digestion. (
A) Unstimulated lung cells harvested from WT mice with and without collagenase digestion and analyzed by FACS. Percentage yield and total number of CD11c
+ cells obtained from resting lung by collagenase digestion (D) are shown compared with those from nondigested tissue (N). (
B) Analysis of non-CD11c
+ cell population from nondigested lung tissue by FACS. Expression of cell markers Mac1, B220, Gr1, γδ, NK, CD4, and CD8 on non-CD11c
+ cells is shown on forward scatter (Fsc) compared with an isotype control. Numbers indicate the percentage of live cells positive for the indicated cell marker.