MyD88-dependent induction of allergic Th2 responses to intranasal antigen
J. Clin. Invest. Damani A. Piggott, et al. 115:459 doi:10.1172/JCI22462 [Go to this article.]

Figure 1
Th2 responses to intranasal protein antigen are MyD88 dependent. (A) BAL inflammatory cells of WT, MyD88-deficient, or TLR4d mice sensitized intranasally with OVA/LPS and WT mice sensitized with PBS. Exposed mice were then challenged 2 weeks later with inhaled OVA. Total bar height represents total BAL cell number, and error bars are based on total cell numbers. Stacked bars represent cell differential counts. n = 4. *P < 0.05, total BAL cell number from WT vs. MyD88-deficient and WT vs. TLR4d. One representative experiment of 4 is shown. (B) OVA-specific serum antibody responses by ELISA after challenge with OVA in WT, MyD88-deficient, and TLR4d mice sensitized as described in A. P < 0.05, WT vs. MyD88-deficient and WT vs. TLR4d, for IgE and IgG1 responses. (C) Cytokine production from lung draining lymph nodes after challenge with OVA in WT, MyD88-deficient, and TLR4d mice sensitized as described in A.