The IL-6R α chain controls lung CD4⁺CD25⁺ Treg development and function during allergic airway inflammation in vivo
J. Clin. Invest. Aysefa Doganci, et al. 115:313 doi:10.1172/JCI22433 [Go to this article.]

Figure 5
IL-10–producing CD4⁺ T cells in the lungs of anti–IL-6R antibody–treated mice. (A and B) CD4⁺ T cells were isolated from the lung of treated or untreated mice and cultured overnight in the presence of anti-CD3 antibodies. CBA was performed on the CD4⁺ T cell supernatants. CD4⁺ T cells isolated from the lung of anti–IL-6R antibody–treated mice secreted increased amounts of IL-10 and IFN-γ as compared to those of OVA-sensitized and -challenged, untreated or IgG-treated mice (P = 0.023 and P = 0.013 for IL-10 and IFN-γ, respectively). Levels of the Th2-type chemokine MCP-1 were not upregulated upon anti–IL-6R antibody treatment, however. (C and D) By contrast, lung CD4⁺ cells isolated from mice treated i.n. with gp130-Fc did not show changes either in IL-10 (C) or IFN-γ production (D).