Organ-specific roles for transcription factor NF-κB in reovirus-induced apoptosis and disease
J. Clin. Invest. Sean M. O’Donnell, et al. 115:2341 doi:10.1172/JCI22428 [Go to this article.]

Figure 4
Inflammation, reovirus protein expression, TUNEL staining, and immunohistochemical detection of activated caspase-3 in the brain of reovirus-infected p50^+/+ (A) and p50^–/– (B) mice. Newborn mice were inoculated perorally with 10⁴ PFU reovirus T3SA⁺. At 12 days after inoculation, brains were harvested, paraffin embedded, sectioned, and stained with H&E, polyclonal reovirus-specific antiserum (Reo), TUNEL, or activated caspase-3–specific antiserum as indicated. Shown are consecutive sections of diencephalon. Original magnification, ×100 (top panels) and ×400 (bottom panels). (C) Newborn mice were inoculated intracranially with 10⁴ PFU T3SA⁺ or gelatin saline (Mock). At 6 days after inoculation, mice were euthanized, and brain sections were stained using a TUNEL assay. Shown are sections of the upper brain stem. Original magnification, ×200. Brown staining indicates reovirus protein, fragmented DNA, or activated caspase-3.