Adoptive immunotherapy of prostate cancer bone lesions using redirected effector lymphocytes
J. Clin. Invest. Jehonathan H. Pinthus, et al. 114:1774 doi:10.1172/JCI22284 [Go to this article.]

Figure 2
Effect of irradiation and cyclophosphamide on SDF-1 mRNA expression in BM and subsequent migration of human T bodies to murine BM. (A and B) SCID mice were irradiated with 2 Gy or injected i.p. with 200 mg/kg of cyclophosphamide or were left untreated. (A) Twenty-four hours after the treatments, mRNA was prepared and subjected to RT-PCR. Cycloph, cyclophosphamide; mSDF-1α, mouse SDF-1α; normal, untreated mouse. H₂O was used as a negative control. (B) Kinetics of the migration of human T bodies to the BM after their i.v. injection into treated mice. The number of T bodies reaching the BM (events) was determined by FACS analysis gating on GFP (n = 3 mice per group; experiment was repeated at least twice). The number of T bodies that accumulated in the BM is significantly larger in irradiated than in nonirradiated recipients at 6 and 12 hours (P = 0.054 and P = 0.011, respectively).