An uncleavable form of pro–scatter factor suppresses tumor growth and dissemination in mice
J. Clin. Invest. Massimiliano Mazzone, et al. 114:1418 doi:10.1172/JCI22235 [Go to this article.]

Figure 3
Delivery of uncleavable SF to tumor cells using lentivirus vectors. (A) MDA-MB-435 cells transduced with the indicated lentivirus vector (empty vector [Empty v.]; uncleavable SF; WT SF) were cultured in the absence (0%) or presence (4%) of FBS, and their conditioned medium was analyzed by Western blot using anti–SF β-chain. (B) Analysis of Met phosphorylation in lentivirus vector–transduced MDA-MB-435 cells. Receptor activation was assessed by Western blot using anti-phosphotyrosine in quiescent (0% FBS) or exponentially growing (4% FBS) cells in the presence (+) or absence (–) of recombinant SF, as indicated. (C) Proliferation rate analysis of lentivirus vector–transduced MDA-MB-435 cells. Cell numbers were assessed every 2 days. (D) In vitro morphology of lentivirus vector–transduced A549 cells stained with crystal violet.