Chronic lymphocytic leukemia B cells contain anomalous Lyn tyrosine kinase, a putative contribution to defective apoptosis
J. Clin. Invest. Antonella Contri, et al. 115:369 doi:10.1172/JCI22094 [Go to this article.]

Figure 3
Expression of Src kinase proteins and Lyn mRNA in normal and CLL B cells. (A) Immunoblot analysis of the cellular protein level of different Src kinases. The lysates obtained from normal B lymphocytes and leukemic B cells from CLL patients were analyzed by immunostaining with antibodies against the indicated Src kinases. Blots were reprobed with anti–β-actin antibody as loading control (the reprobing of the Lyn blot is shown). The panel is representative of Lyn analysis performed with 3 different tonsils, 5 peripheral blood samples, and 40 B-CLL samples. The analysis of the other Src kinases was performed with 10 CLL samples. (B) The analysis of Lyn immunolocalization was performed by confocal microscopy in 3 different normal and 10 B-CLL samples. Original magnification, ×60. (C) The analysis of Lyn and β-actin mRNA expression was performed by Northern blotting in 16 B-CLL samples and the same normal B cells shown in A. Data from 8 representative B-CLL patients are shown.