Chronic lymphocytic leukemia B cells contain anomalous Lyn tyrosine kinase, a putative contribution to defective apoptosis
J. Clin. Invest. Antonella Contri, et al. 115:369 doi:10.1172/JCI22094 [Go to this article.]

Figure 2
Effect of several inhibitors on CLL cellular tyrosine phosphorylation and tyrosine kinase activities. Freshly isolated B cells obtained from CLL patients no. 19 and no. 40 were cultured for 15 minutes without or with the indicated tyrosine kinase inhibitors. (A) Cells were lysed and analyzed by immunostaining with antibody against phosphorylated tyrosine as in Figure 1. The molecular mass (kDa) of protein standards is indicated in the center. (B and C) Cells were lysed, and the tyrosine kinase activity was detected either on the Src-specific peptide substrate cdc2(6–20) (B) or on the nonspecific random polymer polyGlu₄Tyr (C). The data are representative of experiments performed with 8 different B-CLL samples. Ctrl, control.