Constitutive activity of the melanocortin-4 receptor is maintained by its N-terminal domain and plays a role in energy homeostasis in humans
J. Clin. Invest. Supriya Srinivasan, et al. 114:1158 doi:10.1172/JCI21927 [Go to this article.]

Figure 5
The N-terminal domain uses a subset of the acidic residues used by α-MSH. (A) The cAMP accumulation was measured in cells transiently transfected with WT and mutated receptors after stimulation with 10 μM α-MSH. (B) The ratio of receptor activation (as measured by cAMP accumulation) to cell-surface expression (as measured by ELISA) was measured in the WT and the mutated receptors.