The unfolded protein response sensor IRE1α is required at 2 distinct steps in B cell lymphopoiesis
J. Clin. Invest. Kezhong Zhang, et al. 115:268 doi:10.1172/JCI21848 [Go to this article.]

Figure 1
Phenotype of ire1α^–/– embryos. (A) Southern blot analysis of genomic DNA from yolk sacs of ire1α^+/+, ire1α^+/–, and ire1α^–/– embryos. The WT and knockout embryos have a 3-kb WT ire1α allele and a 4-kb recombinant allele, respectively. The heterozygous embryo has both the WT and the recombinant alleles. Rec. allele, ire1α recombinant allele; WT allele, WT ire1α allele. (B) Morphology of ire1α^–/–, ire1α^+/–, and ire1α^+/+ embryos. The ire1α^–/–, ire1α^+/–, and ire1α^+/+ embryos were from the same littermates at E12.5. Magnification, ×10. (C) Histological analysis of ire1α^–/–, ire1α^+/–, and ire1α^+/+ embryos at E12.5. Paraffin-embedded sections (5 μM) of embryos were stained with H&E and visualized at magnification ×20.