Cyclic nucleotide phosphodiesterase 3A–deficient mice as a model of female infertility
J. Clin. Invest. Silvia Masciarelli, et al. 114:196 doi:10.1172/JCI21804 [
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Figure 3Histology of ovaries from
Pde3a+/+ and
Pde3a –/– mice. (
A–
H) H&E sections of ovaries harvested from C57BL/6J-129/SvJ females (3 months old),
Pde3a+/+ (
A and
C) or
Pde3a –/– (
B and
D), or from females (5 weeks old) after induction of superovulation as described in Methods,
Pde3a+/+ (
E and
G) or
Pde3a –/– (
F and
H). Magnification,
A,
B,
E, and
F: ×4;
C,
D,
G, and
H: ×20.
Pde3a–/– females exhibited normal ovaries and follicles, either in normal condition or following superovulation. (
I–
L)
Pde3a+/+ or
Pde3a–/– females were injected with PMSG and hCG as described in Methods; 9 hours after injection with hCG, H&E sections were prepared. Note the expanded cumulus, which has lost contact with the follicle wall in both genotypes. Whereas the
Pde3a+/+ oocytes are in metaphase (with an evident spindle) (
I and
J), the immature
Pde3a–/– oocytes exhibit an intact nuclear membrane (GV stage) (
K and
L).
