Distinct contribution of IL-6, TNF-α, IL-1, and IL-10 to T cell–mediated spontaneous autoimmune arthritis in mice
J. Clin. Invest. Hiroshi Hata, et al. 114:582 doi:10.1172/JCI21795 [Go to this article.]

Figure 2
Expression of cytokines at mRNA and protein levels. (A) Quantitative RT-PCR for indicated genes was performed with ankle joints from 8- or 24-week-old SKG or BALB/c mice. Bars show the means ± SD. See Methods for the definition of units. (B) Concentrations of indicated cytokines, assessed by ELISA, in the joint fluid of 32-week-old SKG and BALB/c mice (n = 10 each). Bars show the means ± SD. (C–F) Immunohistochemical staining of the synovial tissue of a finger joint of a 4-month-old SKG mouse for IL-1β (C), TNF-α (D), or IL-6 (E), with staining control (F) (original magnification, ×40). Insets show higher magnifications of a part of synovium.