T cell–mediated vascular dysfunction of human allografts results from IFN-γ dysregulation of NO synthase
J. Clin. Invest. Kian Peng Koh, et al. 114:846 doi:10.1172/JCI21767 [
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Figure 1Effects of allogeneic T cells on arterial graft function at 1 week in vivo. Transplanted human arterial segments were recovered from mice injected with saline (open squares) or PBMCs (filled squares) 7–9 days before harvest (
n = 5 pairs from four experiments). (
A–
D) Response curves. Restriction response to various concentrations of PGF
2α (
A) and relaxation response curves for nitroprusside (
B), bradykinin (
C), or substance P (
D) after preconstriction with PGF
2α. *
P < 0.05; **
P < 0.01;
#P < 0.001 vs. saline control. mN, milliNewtons. (
E) Immunohistochemistry of graft sections stained for human and murine (inset) CD31, human CD45, and HLA-DR. The staining for human CD45 is indistinguishable from that for human CD3 (data not shown). Original magnification, ×200.
