Increased DC trafficking to lymph nodes and contact hypersensitivity in junctional adhesion molecule-A–deficient mice
J. Clin. Invest. Maria Rosaria Cera, et al. 114:729 doi:10.1172/JCI21231 [Go to this article.]

Figure 5
JAM-A expression and in vitro DC migration. (A) Chemotaxis of iDCs and mDCs was evaluated using the Transwell system. Data are presented as percent migration, calculated as described in Methods. Data are mean ± SEM of triplicates from a typical experiment out of three performed. (B) Basal transmigration of Jam-A^+/+ and Jam-A ^–/– mDCs across monolayers of vascular (1G11 cells) or lymphatic (MELCs) endothelial cells grown on the upper or lower side (reversed MELCs) of the filter (see Methods). Data, presented as percent transmigration, are mean ± SEM of triplicates from a typical experiment out of three performed. *P < 0.01 by paired Student’s t test comparing transmigration of Jam-A^+/+ and Jam-A ^–/– mDCs through MELCs. (C) Distribution of particle-free areas in random migration. Each bar represents the percentage of cells that have migrated over the indicated particle area in one representative experiment out of three performed. In this experiment the mean ± SEM of the particle area covered by migrating cells was 378 ± 46 μm² for Jam-A^+/+ iDCs (n = 25 cells), 1,511 ± 249 μm² for Jam-A ^–/– iDCs (n = 20 cells), 541 ± 79 μm² for Jam-A^+/+ mDCs (n = 17 cells), and 1,448 ± 217 μm² for Jam-A ^–/– mDCs (n = 14 cells).