Increased DC trafficking to lymph nodes and contact hypersensitivity in junctional adhesion molecule-A–deficient mice
J. Clin. Invest. Maria Rosaria Cera, et al. 114:729 doi:10.1172/JCI21231 [Go to this article.]

Figure 4
Analysis of lymph nodes. (A) FACS analysis of different populations of cells from inguinal lymph nodes from Jam-A^+/+ and Jam-A^–/– mice (percentage and MFI). (B) DC subsets were characterized in peripheral lymph nodes from Jam-A^+/+ and Jam-A^–/– mice. CD11c⁺ DCs were gated and stained for CD11b and CD8α. Myeloid DCs (M-DCs) are expressed as the percentage of CD11b/⁺/CD8α^–, and lymphoid DCs (L-DCs) are expressed as the percentage of CD11b^–/CD8α⁺DCs within the CD11c⁺ population. Plasmacytoid DCs (P-DCs) were identified as B220⁺ cells within a CD11c⁺/CD11b^– gated population. The same number of plasmacytoid DCs was found in Jam-A^+/+ and Jam-A^–/– mice when B220⁺/GR1⁺ DCs were considered to be part of the CD11c⁺/CD11b^– gated population.