Increased DC trafficking to lymph nodes and contact hypersensitivity in junctional adhesion molecule-A–deficient mice
J. Clin. Invest. Maria Rosaria Cera, et al. 114:729 doi:10.1172/JCI21231 [Go to this article.]

Figure 3
Characterization of DCs from Jam-A^+/+ and Jam-A ^–/– mice. CD34⁺ bone marrow precursor cells were used to generate iDCs and mDCs in vitro, and these were functionally characterized as detailed in Methods. (A) JAM-A expression on Jam-A^+/+ and Jam-A ^–/– iDCs and mDCs. Unlabeled lines correspond to staining with an irrelevant mAb of an identical isotype. (B) Membrane phenotype of iDCs and mDCs from Jam-A^+/+ and Jam-A ^–/– mice. Percentage and mean fluorescence intensity (MFI) of positive cells are shown. (C) Mixed lymphocyte response performed in the presence of different numbers of mDCs from Jam-A^+/+ and Jam-A ^–/– mice. mDCs are most effective in mixed lymphocyte response, and for simplicity only data with mDCs are shown in the Figure. iDCs from Jam-A^+/+ and Jam-A ^–/– mice did not differ in inducing mixed lymphocyte response (data not shown). (D) Uptake of FITC-dextran as evaluated by FACS analysis. Data in B are of one representative experiment out of five performed. Data in C and D are mean ± SEM of one representative experiment out of three performed in triplicate.