Increased DC trafficking to lymph nodes and contact hypersensitivity in junctional adhesion molecule-A–deficient mice
J. Clin. Invest. Maria Rosaria Cera, et al. 114:729 doi:10.1172/JCI21231 [
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Figure 1Characterization of
Jam-A –/– and endothelial
Jam-A –/– mice. (
A) The targeted allele containing 3 LoxP sites (
flox/flox), the excised allele (
Jam-A –/–), and the WT allele (
Jam-A+/+) are shown. (
B) Genomic PCR of murine tail biopsies. To identify
Jam-A –/– mice obtained from interbreeding of heterozygous mice, the combination of CAG–Cre-F, CAG–Cre-R, TS379, TS512, TS447, and TS444 LoxP primers was used. To identify endothelial
Jam-A –/– mice, the combination of Tie-2 Cre, TS379, and TS512 primers was used. TS379 and TS512 generate a 500-bp product for minus allele, an 800-bp product for WT allele, and a 3,000-bp product (absence of band) for flox allele. (
C) FACS analysis of JAM-A on mouse leukocytes isolated from peritoneal cavities 24 hours after injection of thioglycollate. (
D) Expression of JAM-A on endothelial cells derived from lungs of
Jam-A+/+ and
Jam-A –/– animals evaluated by FACS, immunoprecipitation, and Western blot analysis with mAb BV12. In
C and
D, gray lines represent negative controls obtained using the secondary Ab alone.
