Cortical spreading depression activates and upregulates MMP-9
J. Clin. Invest. Yasemin Gursoy-Ozdemir, et al. 113:1447 doi:10.1172/JCI21227 [
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Figure 8CSD-induced plasma protein leakage was suppressed after MMP inhibition or after CSD in mice lacking
MMP-9 gene expression. Evans blue leakage is blocked by the nonselective metalloprotease inhibitor GM6001 (65 mg/kg intraperitoneally, given 1 hour before and 4 hours after CSD) when examined at 12 hours, compared with that of vehicle-treated animals (
n = 5 per group; *
P < 0.05 vs. vehicle). A decrease in leakage 6 hours after CSD was detected in mice lacking
MMP-9 gene expression compared with that of the same age-matched mice of similar background (CD-1) (
n = 5 per group; #
P < 0.05 vs. wild-type strain), although CSD itself did not differ between strains. Data are expressed as a ratio of Evans blue leakage in homogenates from both sides.
