FLI1 monoallelic expression combined with its hemizygous loss underlies Paris-Trousseau/Jacobsen thrombopenia
J. Clin. Invest. Hana Raslova, et al. 114:77 doi:10.1172/JCI21197 [Go to this article.]

Figure 5
Single-cell RT-PCR analysis of FLI1 expression in peripheral blood CD34⁺ cells from three controls and two patients, cultured in presence of TPO, IL-3, IL-6, SCF, and FLT3-L. On day 4, the CD34⁺CD41 ^–CD42 ^–, CD34⁺CD41⁺CD42 ^–, and CD34⁺CD41⁺CD42⁺ populations were sorted after immunolabeling at one cell per well in a 96-well plate. Single-cell RT-PCR was performed on 100 individual cells in each population. β2-M was used as an internal control of mRNA integrity, and only cells positive for β2-M were analyzed. (A) The graphs represent the number of FLI1–expressing cells out of 100 analyzed cells from different populations of three healthy individuals (Control) (the error bars represent the SD of the mean of three independent experiments) and of two Paris-Trousseau patients. (B) RT-PCR analysis in a representative 18 (of 100) CD34⁺CD41⁺CD42^– cells from one healthy individual and two patients. Safety control of experiment was performed in absence of sorted cell (lane 20: Control).