FLI1 monoallelic expression combined with its hemizygous loss underlies Paris-Trousseau/Jacobsen thrombopenia
J. Clin. Invest. Hana Raslova, et al. 114:77 doi:10.1172/JCI21197 [Go to this article.]

Figure 1
Comparison of FLI1 and ETS1 expression in different human hematopoietic lineages: NK cells, lymphocytes T and B (LyT and LyB), and MKs. The absolute expression level of FLI1 and ETS1 in 100 ng of total cell RNA was measured relative to different dilutions (corresponding to 10²–10⁶ copies) of plasmids harboring either FLI1 or ETS1 cDNA. (A) The graph represents the relative expression between FLI1 and ETS1 in NK (CD56), T (CD3), and B (CD19) cells and in MKs. The value indicated above or under the columns is the ratio between the FLI1 and the ETS1 copy numbers in cDNA prepared from 100 ng of total cell RNA. (B) The absolute expression level of FLI1 and ETS1 in MKs measured as the number of mRNA copies per 100 ng of total RNA. The error bars represent the SD of the mean of three experiments, each performed in triplicate wells.