Structural and functional impairment of endocytic pathways by retinitis pigmentosa mutant rhodopsin-arrestin complexes
J. Clin. Invest. Jen-Zen Chuang, et al. 114:131 doi:10.1172/JCI21136 [
Go to this article.]
Figure 6The altered endocytic activity of Tf and LDL in cells that expressed R135L/v-arr. Cells transfected with R135L/GFPv-arr (
A and
B,
E and
F,
I and
J,
M and
N) and WT rhodopsin/GFPv-arr (
C and
D,
G and
H,
K and
L,
O and
P) were incubated with Alexa 594–conjugated Tf at 37–C for 5 minutes (
A and
B,
C and
D), for 2 hours (
E and
F,
G and
H), or for 2 hours plus a 30-minute chase (
I and
J,
K and
L) before fixation and visualization. Alternatively, cells were treated with DiI-LDL for 2 minutes followed by a 28-minute chase before the fixation (
M and
N,
O and
P). Confocal images of GFPv-arr (green) and internalized Tf (red) or LDL (red) are shown. In
J and
L, the DAPI nuclear labeling demonstrated that the nontransfected cells (in
J and
L) and WT/GFPv-arr–transfected cells (in
L) have no Tf signals, as the Tf was completely expelled from cells. Scale bars: 20 μm.
