Apolipoprotein A-I is a selective target for myeloperoxidase-catalyzed oxidation and functional impairment in subjects with cardiovascular disease
J. Clin. Invest. Lemin Zheng, et al. 114:529 doi:10.1172/JCI21109 [Go to this article.]

Figure 1
ApoA-I is a preferred target for nitration in serum. Serum samples (25 μg total protein per lane) from 3 healthy controls and 3 subjects with CVD were separated by SDS-PAGE. The gels (12.5%) were either stained for protein with Coomassie blue (A) or transferred and probed with a mAb specific for protein-bound NO₂Tyr (B). The apoA-I bands were identified by sequence analysis by tandem mass spectrometry (MS). The disproportionate recognition of the apoA-I band in the Western blot analysis is consistent with an enhanced NO₂Tyr content of that protein.