A novel transgenic mouse model for immunological evaluation of carcinoembryonic antigen–based DNA minigene vaccines
J. Clin. Invest. He Zhou, et al. 113:1792 doi:10.1172/JCI21107 [Go to this article.]

Figure 4
DNA minigene–induced cytotoxicity is mediated by CD8⁺ T cells. Groups of C57BL/6-CEA-A2Kb mice (n = 4) were immunized three times at 2-week intervals with attenuated S. typhimurium harboring either pHI-CAP1-6D or pHI-691 vectors. Mice were sacrificed 2 weeks after the last immunization, and isolated splenocytes were fractionated into CD8⁺ or CD8^– populations. Each cell population was stimulated with irradiated MC-38-CEA-A2Kb cells for 5 days. Thereafter, cytotoxicity assays were performed with CEA₆₉₁-loaded T2 cells as targets. (A) Purity of each subpopulation after MACS. (B) Cytotoxicity of each cell population at E/T ratio of 20:1. (C) Cytotoxicity of CD8⁺ populations is shown at different E/T ratios.