A novel transgenic mouse model for immunological evaluation of carcinoembryonic antigen–based DNA minigene vaccines
J. Clin. Invest. He Zhou, et al. 113:1792 doi:10.1172/JCI21107 [Go to this article.]

Figure 2
An HLA-A2–restricted, CEA₆₉₁-specific response is induced by the pHI-691 DNA minigene vaccine. Groups of C57BL/6-CEA-A2Kb mice (n = 4) were immunized three times at 2-week intervals with attenuated S. typhimurium harboring the vectors indicated. Two weeks after the last immunization, mice were sacrificed and ELISPOT assays performed on splenocytes isolated by using synthetic peptides (10 ∝g/ml) as stimulators (A). The remaining splenocytes were stimulated with irradiated MC-38-CEA-A2Kb cells for 5 days, and ELISPOT assays were then performed using either irradiated unloaded or peptide-loaded T2 cells as stimulators (B). (C) HLA-A2 expression of T2 (left) and B3 (right) cells. B3 is an EBV-transformed cell line generated from a healthy HLA-A2⁺ individual as described in Methods. Cells were treated with control antibody (thin dashed lines) or anti–HLA-A2 antibody (thick solid lines) and then stained with PE-conjugated goat anti–mouse Ig.