A novel transgenic mouse model for immunological evaluation of carcinoembryonic antigen–based DNA minigene vaccines
J. Clin. Invest. He Zhou, et al. 113:1792 doi:10.1172/JCI21107 [Go to this article.]

Figure 1
Expression vectors are constructed and verified. (A) Schematic map of vector constructs. Minigenes encoding HIVtat translocation peptide, a spacer, and human CEA epitopes CAP1-6D or CEA₆₉₁ were assembled by PCR with overlapping oligonucleotides as templates. The PCR fragments generated were cloned into a pCMV vector by using BssHII and XhoI restriction sites. (B) Proteins encoded by minigenes were expressed in mammalian cells. This expression was indicated when 293T cells were transfected with either pHI-myc or pHI-691-myc for 24 hours, harvested, lysed, and analyzed by Western blotting with monoclonal antibody against myc.