MMPs are required for recruitment of antigen-nonspecific mononuclear cells into the liver by CTLs
J. Clin. Invest. Giovanni Sitia, et al. 113:1158 doi:10.1172/JCI21087 [Go to this article.]

Figure 5
Enhanced expression of TIMP-1 does not affect the recruitment of PMNs into the liver. (A) Intrahepatic analysis of Gr-1⁺/CD11b⁺/CD11c^– cells in the same animals described in the legend to Figure 3. Livers were weighed at the time of autopsy. Total IHLs were isolated from two liver lobes of a known weight and analyzed by flow cytometry. The indicated numbers represent the percentage of Gr-1⁺/CD11b⁺/CD11c^– cells detected in the total IHL population isolated from the whole liver. (B) Total liver MPO activity was determined as described in the Methods in the same mice. The indicated numbers are displayed as fold induction over NaCl-injected controls (set as 1), and they represent the mean of six mice per group. Total hepatic RNA from the same mice was also analyzed by RPA to measure the intrahepatic content of MMP-8 (C) and MMP-9 (D) mRNAs. The indicated numbers, calculated by phosphor imaging analysis, are displayed as fold induction over NaCl-injected controls (set as 1). The numbers were obtained by dividing each MMP value by the amount of the corresponding housekeeping gene L32 RNA, and they represent the mean of six mice per group.