MMPs are required for recruitment of antigen-nonspecific mononuclear cells into the liver by CTLs
J. Clin. Invest. Giovanni Sitia, et al. 113:1158 doi:10.1172/JCI21087 [
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Figure 2Hydrodynamic injection (inj.) of a plasmid encoding murine TIMP-1 leads to enhanced expression of TIMP-1 RNA in the liver and TIMP-1 protein in the serum. Five groups of age- and HBeAg-matched female HBV transgenic mice (three mice per group) were hydrodynamically injected with pcDNA3.1-TIMP-1. Mice were bled and sacrificed, and the livers were harvested at days 1, 2, 3, 4, and 5 after hydrodynamic injection. The intrahepatic content of pcDNA3.1-TIMP-1 DNA and TIMP-1 RNA were measured by Southern blot and RPA, respectively (upper panels), and the serum levels of TIMP-1 protein were measured by ELISA (lower panel). The mean sALT activity (± SD), measured at the time of autopsy, is indicated for each group and is expressed in units/liter.
