Preclinical deposition of pathological prion protein PrPSc in muscles of hamsters orally exposed to scrapie
J. Clin. Invest. Achim Thomzig, et al. 113:1465 doi:10.1172/JCI21083 [
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Figure 4Testing of bioassay reporter animals for cerebral PrP
Sc deposition. Western blot detection of PrP27-30, the protease-resistant core of PrP
Sc, in proteinase K–digested brain homogenates from hamsters intracerebrally inoculated with muscle samples from terminally ill donors orally challenged with 263K scrapie. Lane 1: representative finding for reporter animals that succumbed to scrapie; sample from a terminally ill hamster challenged with homogenized M. biceps femoris (hindlimb) containing 10
–6 g brain tissue. Lane 2: representative finding for reporter animals that failed to show scrapie symptoms until 350 days after infection; sample from a subclinically infected hamster challenged with homogenized M. triceps brachii (forelimb) containing 10
–3 g brain tissue.
