Kinase suppressor of Ras-1 protects intestinal epithelium from cytokine-mediated apoptosis during inflammation
J. Clin. Invest. Fang Yan, et al. 114:1272 doi:10.1172/JCI21022 [Go to this article.]

Figure 2
Loss of KSR1 in colon epithelium inhibits TNF-stimulated cell survival signals in vivo. KSR1^+/+ or KSR1^–/– mice were injected with TNF or PBS for the indicated times. Total colon mucosal lysates were collected for Western blot analysis with anti-KSR1, anti–P-ERK1/2, anti–P-IκBα, anti–P-Akt, and anti-ERK1/2 antibodies (A). P, phosphorylation. Paraffin-embedded colon tissues were immunostained with anti–NF-κB p65 subunit (B) or cIAP2 (C), labeled with peroxidase, and visualized using DIC microscopy (magnification, ×40). Arrows indicate nuclear localization of NF-κB (B) and cIAP2-positive cells (C). Shown is 1 representative of 5 mice in each treatment group.