Nef stimulates proliferation of glomerular podocytes through activation of Src-dependent Stat3 and MAPK1,2 pathways
J. Clin. Invest. John Cijiang He, et al. 114:643 doi:10.1172/JCI21004 [Go to this article.]

Figure 3
Cell-cell contact does not inhibit Nef-induced Stat3 and MAPK1,2 activation. Nef-infected (Nef) or control vector–infected (V) podocytes were prepared as described. Cells grown to 50% confluence (Non-C) were compared with those grown to 100% confluence (Confluent). Western blot was performed for phospho-Stat3, total Stat3 (T-Stat3), phospho-MAPK1,2, and total MAPK1,2 (T-MAPK1,2) using their specific antibodies as described. Densitometric data: phospho-MAPK1,2/total MAPK1,2 ratio: V = 0.66 ± 0.13, Nef = 1.21 ± 0.19 for nonconfluent cells; V = 0.67 ± 0.18, Nef = 1.24 ± 0.12 for confluent cells; phospho-Stat3/total Stat3 ratio: V = 0.65 ± 0.11, Nef = 1.26 ± 0.16 for nonconfluent cells; V = 0.45 ± 0.10, Nef = 1.14 ± 0.26 for confluent cells; P < 0.05 when Nef is compared with V, n = 3.