Circulating fibrocytes traffic to the lungs in response to CXCL12 and mediate fibrosis
J. Clin. Invest. Roderick J. Phillips, et al. 114:438 doi:10.1172/JCI20997 [
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Figure 1Characterization of human fibrocytes and their trafficking to bleomycin-induced lung fibrosis in SCID mice. (
A) Human fibrocytes stained with control Ab, human Col I, human CXCR4, and human α-SMA 3 weeks after purification. Representative fields viewed at ×400 magnification. (
B) Isolated human fibrocytes triple-stained for Col I, CD45, and CXCR4 were examined by FACS analysis. Data shows CD45
+ fibrocytes that were examined for dual expression of CXCR4 and Col I. (
C) Isolated human fibrocyte chemotaxis in response to 0, 30, and 100 ng/ml of CXCL12. Data are representative of three experiments. *
P < 0.05. h.p.f., high-powered field. (
D) Isolated human fibrocytes (10
6) were injected into the tail vein of SCID mice at day 4 after treatment with either intratracheal bleomycin (Bleo) or saline, followed by recovery of cells from the lungs at day 8. Cells were then stained for human CD45, Col I, and CXCR4 and analyzed by flow cytometry to determine trafficking of human fibrocytes to the lungs of SCID mice. *
P < 0.05.
