Specific induction of neuronal cells from bone marrow stromal cells and application for autologous transplantation
J. Clin. Invest. Mari Dezawa, et al. 113:1701 doi:10.1172/JCI20935 [Go to this article.]

Figure 4
Transcription factors during induction. (A) RT-PCR of rat MSCs, N-MSCs, 24 hours and 5 days after trophic factor induction in TF-MSCs, and positive control. (B) Western blot of Notch extracellular domain (NECD) and NICD in rat MSCs, N-MSCs, TF-MSCs (5 days after trophic factor induction), and positive control from brain. β-tubulin (tub) as a loading control. (C and D) Generation of MAP-2ab⁺ cells (green in D) by JAK/STAT inhibitor WHI-P131 administration and subsequent trophic factor induction in rat MSCs. (E and F) Rat MSCs cotransfected with active form of STAT3 (anti-FLAG; green) and NICD, followed by trophic factor induction. Only a small number of cells were positive for MAP-2ab (red). (G and H) Hes1/5 transfection to rat MSCs. Bars in C–H = 80 ∝m.