Specific induction of neuronal cells from bone marrow stromal cells and application for autologous transplantation
J. Clin. Invest. Mari Dezawa, et al. 113:1701 doi:10.1172/JCI20935 [Go to this article.]

Figure 3
Analysis of TF-MSCs (5 days after trophic factor induction). (A–C) Phase contrast of TF-MSCs from rats (A and B) and humans (C). Bars, A = 200 ∝m, B and C = 50 ∝m. (D–F and H–J) Immunocytochemical analysis of neuronal and glial markers in rat (F, H–J) and human (D and E) TF-MSCs. MAP-2ab (D), neurofilament-M (E), and β-tubulin isotype 3 (F) were detected. None of the cells were reactive to GFAP (H), GalC (I), and O4 (J). (G) The Brd-U labeling of rat TF-MSCs. MAP-2ab–positive cells (green) did not incorporate Brd-U (red), whereas negative cells were occasionally incorporated with Brd-U. Bars in D–J = 100 ∝m. (K) Western blot analysis of MAP-2ab and GFAP rat samples. Brain, positive control; TF-MSC. β-tubulin (tub) as a loading control. (L–Q) Patch clamp. K⁺ current increased with trophic factor induction up to approximately 1,600 pA and 4,000 pA in rat (L) and human (M) TF-MSCs, respectively. (N) Phase contrast of human TF-MSCs recorded in (M). (O) Voltage-gated inward current recorded in rat BDNF + NGF–treated TF-MSCs. A series of Na current to show the process of block by TTX. Capacity current was blanked. (P) Action potentials from rat BDNF + NGF–treated TF-MSCs; subthreshold, threshold, and suprathreshold current injections were made. (Q) Immunocytochemistry of sodium channel (green). Bar = 30 –m. (R) Relative promoter activities of NeuroD and GFAP in rat MSCs, N-MSCs, and TF-MSCs.