Specific induction of neuronal cells from bone marrow stromal cells and application for autologous transplantation
J. Clin. Invest. Mari Dezawa, et al. 113:1701 doi:10.1172/JCI20935 [Go to this article.]

Figure 1
Characterization of isolated MSCs. (A) FACS analysis of rat MSCs. Numbers in panels represent mean fluorescent intensity of the cells expressing each marker. (B and C) Phase-contrast microscopy of rat (B) and human (C) MSCs. (D–K) Immunocytochemistry of CD29 (D), CD90 (E), and CD34 (F) of human MSCs and PDGF receptor β (PDGFR) (G), and smooth muscle actin (sm-actin) (H), CD31 (I), CD45 (J), and neurofilamen-M (K) of rat MSCs. Bars = 50 ∝m. (L–P) Adipogenic (L and M), chondrogenic (N), and osteogenic (O) induction from human (L–N) and rat (O) MSCs. Micrographs in (M) show oil red staining of lipid droplet in adipocytes in (N) immunocytochemistry of collagen type II of chondrogenic induction and in (O) alkaline phosphatase in osteocytes. (L) Phase-contrast image of adipocytes. Bars = 50 ∝m. (P) Alkaline phosphatase (ALP) activity of rat and human MSCs before and after osteogenic induction. **P < 0.01.