Increased postischemic brain injury in mice deficient in uracil-DNA glycosylase
J. Clin. Invest. Matthias Endres, et al. 113:1711 doi:10.1172/JCI20926 [Go to this article.]

Figure 4
(A and B) Ung mRNA and (C) activity in different mouse tissues and in brain after filamentous MCAo. (A) Steady-state Ung mRNA expression was measured by Northern blotting according to standard techniques in several mouse organs. Expression in the brain is detectable, although at lower levels, compared with other organs. The blot is representative of three different experiments. (B) Ung mRNA after 30 minutes of MCAo was significantly upregulated over time starting at 6 hours of reperfusion with a maximum of 36 hours of reperfusion. Band intensities were quantified by densitometry and values are mean ± SEM of Ung/β-actin expression ratios from three independent experiments. Expression at 36 hours is set as 100%. (C) Uracil-DNA glycosylase activity was measured in brain extracts (nuclear and cytosolic/mitochondrial fractions, respectively) of sham-operated control mice (cont.) and at different time points after 30 minutes of MCAo.