Increased postischemic brain injury in mice deficient in uracil-DNA glycosylase
J. Clin. Invest. Matthias Endres, et al. 113:1711 doi:10.1172/JCI20926 [
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Figure 2Ung
–/– MEFs are susceptible to nitric oxide toxicity. (
A)
Ung–/– and
Ung+/+ MEF were subjected to different doses of SNP for 24 hours, which is known to induce deaminative damage. After 48 hours, viable cells were counted by trypan blue staining (mean ± SE of determinations made in three different experiments; *
P < 0.05 and **
P < 0.01 compared with the values in
Ung+/+ MEF exposed to SNP; ANOVA followed by Scheffe’s post hoc test). (
B) Increased uracil content in DNA in
Ung–/– MEF after exposure to SNP. DNA was prepared from
Ung–/– and
Ung+/+ MEF subjected to different doses of SNP, enzymatically hydrolyzed to free nucleosides, and subjected to reverse-phase HPLC. Ratios of deoxyuridine (dUridine) to deoxycytidine (dCytidine) were determined. Values are mean ± SE from three independent experiments measured in duplicate; ANOVA followed by Scheffe’s post hoc test. ***
P < 0.005.
