Expanded B cell population blocks regulatory T cells and exacerbates ileitis in a murine model of Crohn disease
J. Clin. Invest. Timothy S. Olson, et al. 114:389 doi:10.1172/JCI20855 [Go to this article.]

Figure 5
Soluble IgA production increased in SAMP1/YitFc versus AKR mice. (A) Representative dot plots of κ (1:100 dilution, FL2) and λ (1:1, FL1) light chain antibody isotype levels within MLN supernatants from AKR mice (n = 2) and SAMP1/YitFc mice (n = 2), measured with a cytometric bead array containing isotype-specific beads with preset FL3 intensities. MLNs were crushed, resuspended in 5 ml, and centrifuged to remove the cell pellet from the tested supernatants. An increase of at least twofold in IgG1 κ, IgA κ, IgM κ, IgA λ, and IgM λ was seen in SAMP1/YitFc versus AKR MLNs, as measured by mean fluorescence intensity of the isotype-specific beads. (B) ELISA detecting IgA antibody concentrations (mean ± SEM) in serum samples collected via cardiac puncture from SAMP1/YitFc mice (n = 12) and wild-type AKR mice (n = 13). (C) Concentrations of IgA (n = 4), IgM (n = 2), and IgG2a (n = 2), measured in triplicate by ELISA, from 3-, 7-, and 11-day anti-CD3–stimulated cocultures of SAMP1/YitFc CD4⁺ T cells and B cells versus AKR CD4⁺ T cells and B cells (105 T cells/well and 105 B cells/well). *Significantly greater than AKR concentrations (P < 0.05).