Expanded B cell population blocks regulatory T cells and exacerbates ileitis in a murine model of Crohn disease
J. Clin. Invest. Timothy S. Olson, et al. 114:389 doi:10.1172/JCI20855 [Go to this article.]

Figure 3
SAMP1/YitFc Treg populations block colitis, but not ileitis, in the CD4⁺ T cell adoptive transfer model. (A) Comparison of adoptively transferred ileitis severity (6 weeks after transfer, mean ± SEM) in SCID recipients (n = 4 in each group) induced by 5 × 105 SAMP1/YitFc total MLN CD4⁺ T cells, αE^–CD4⁺ T cells, αE⁺CD4⁺ T cells, or combination treatment using αE⁺CD4⁺ T cells injected 3 weeks (3wk) before, at the same time as, or 3 weeks after αE^–CD4⁺ T cells. (B) In a separate cohort of SCID mice, severity of ileitis induced by 5 × 105 SAMP1/YitFc CD4⁺ T cells (n = 4) was not decreased by coinjection of 5 × 105 AKR MLN CD4⁺ T cells (n = 5). Total inflammatory scores represent the sum of three individual histological indices, including active inflammation, chronic inflammation, and villus architectural distortion. (C) In a third cohort, adoptively transferred ileitis and colitis severities were compared among SCID mice 6 weeks after mice received 5 × 105 SAMP1/YitFc MLN unfractionated CD4⁺, CD45RBhiCD4⁺, CD45RBloCD4⁺, CD25^–CD4⁺, or CD25⁺CD4⁺ T cells. Because villus distortion is not measured in colitis, the sum of active and chronic inflammatory scores was used for this comparison. Data are expressed as mean ± SEM. *Significantly decreased (P < 0.05) compared with ileitis severity in mice receiving αEβ7^–CD4⁺ cells. #Significantly increased compared with colitis severity in mice receiving unfractionated CD4⁺ T cells.