Expanded B cell population blocks regulatory T cells and exacerbates ileitis in a murine model of Crohn disease
J. Clin. Invest. Timothy S. Olson, et al. 114:389 doi:10.1172/JCI20855 [Go to this article.]

Figure 2
SAMP1/YitFc MLN αEβ7⁺CD4⁺ T cells possess a regulatory phenotype. (A) Comparison of the percentage of MLN cells, determined by flow cytometry, that express high levels of CD69 (n = 9 mice), CD25 (n = 9), L-selectin (n = 14), and CD45RB (n = 8) within the αEβ7⁺CD4⁺ and αEβ7^–CD4⁺ T cell subsets. (B) Levels of secreted TNF-α, IL-2, and IL-10, measured by cytometric bead array in triplicate, from 48-hour cultures of SAMP1/YitFc MLN unfractionated CD4⁺ T cells (Total CD4⁺), αE⁺CD4⁺ T cells, or αE^–CD4⁺ T cells (105 cells/well), stimulated with immobilized anti-CD3 and soluble anti-CD28. Values represent the averages of three independent experiments for each group. *Significantly different (P < 0.05) compared to αEβ7^–CD4⁺ T cell population.