Expanded B cell population blocks regulatory T cells and exacerbates ileitis in a murine model of Crohn disease
J. Clin. Invest. Timothy S. Olson, et al. 114:389 doi:10.1172/JCI20855 [Go to this article.]

Figure 1
Expanded B cell and α_Eβ₇⁺CD4⁺ T cell populations in SAMP1/YitFc versus AKR MLN. (A) Total lymphocyte numbers (mean ± SEM) presented as the percentage of total cells in AKR and SAMP1/YitFc MLNs — as determined by lymphocyte-gated flow cytometry — that are CD4⁺ T cells (n = 16 and 32, respectively), CD8⁺ T cells (n = 10 and 19, respectively), and B cells (n = 13 and 24, respectively). Fold increases in the overall size of each of these populations in SAMP1/YitFc versus AKR are indicated. (B) Top, CD4⁺ T cell–gated histograms of β₇ integrin chain expression on MLN cells from SAMP1/YitFc and AKR mice, showing that SAMP1/YitFc mice have an increased percentage of CD4⁺ T cells expressing high levels of β₇. Bottom, the β₇hi cells express β₇ as a dimer with the α_E integrin chain, as α_E⁺ cells display a 1:1 correlation of αE to β7 expression in β7 versus αE dot plots that is not seen in isotype controls. (C) Comparison of the percentage (mean ± SEM) of MLN CD4⁺ T cells that are αE⁺ in SAMP1/YitFc mice (n = 31) versus AKR mice (n = 21). *Significantly greater (P < 0.05) than AKR cell percentage.