Adenovirus-mediated chronic “hyper-resistinemia” leads to in vivo insulin resistance in normal rats
J. Clin. Invest. Hiroaki Satoh, et al. 114:224 doi:10.1172/JCI20785 [Go to this article.]

Figure 7
Decreased AMPK phosphorylation in skeletal muscle, liver, and adipose tissue of hyper-resistinemic rats. Insulin clamp–stimulated skeletal muscle, adipose tissue, and liver samples were collected and homogenized as described in Methods. After fractionation of the resulting homogenates (50 μg) by 10% SDS-PAGE, proteins were transferred to PVDF membranes. Membranes were probed with phosphoryolated AMPK (pAMPK) (Thr172) and AMPK antibodies. The bar graphs show data quantification by NIH Image for results in skeletal muscle, adipose tissue, and liver. Values are means ± SEM of three independent experiments using six animals for each group. Data are expressed relative to control Adv-lacZ values, which were set at 100%. *P < 0.01.