TLR2 is mobilized into an apical lipid raft receptor complex to signal infection in airway epithelial cells
J. Clin. Invest. Grace Soong, et al. 113:1482 doi:10.1172/JCI20773 [
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Figure 5Mobilization of TLR2 and asialoGM1 in response to bacteria or bacterial ligands. Flow cytometry was used to quantify exposed asialoGM1, TLR2, and TLR4 on primary (HNP) cells (
A) or 16HBE cells (
B) after stimulation with
P. aeruginosa or
S. aureus or with monoclonal anti-asialoGM1, Pam
3Cys-Ser-Lys
4 (Pam3Cys), a TLR2 ligand, or LPS, a TLR4 ligand. Peaks outlined with a thin black line indicate binding by secondary antibody alone; gray-shaded peaks represent the labeled population under control conditions; and peaks demarcated by the heavy black line represent the population after stimulation.
