Antagonistic antibody prevents toll-like receptor 2–driven lethal shock-like syndromes
J. Clin. Invest. Guangxun Meng, et al. 113:1473 doi:10.1172/JCI20762 [Go to this article.]

Figure 5
TLR2 expression ex vivo immediately after primary cell isolation. Flow cytometry of splenocytes and peritoneal washout cells from wild-type (TLR2^+/+) and TLR2^–/– mice ex vivo immediately after isolation (n = 5, cells pooled for each sample). (A) CD11b⁺ splenocytes from mice challenged with LPS for 24 hours were analyzed for surface and intracellular TLR2 expression by staining with T2.5 (b line, TLR2^+/+; filled area, TLR2^–/–). (B and C) For analysis of TLR2 regulation upon infection, mice were either left uninfected (–) or infected with B. subtilis and sacrificed after 24 hours (+). Upon staining of CD11b, cells were stained with T2.5 (TLR2) either without permeabilization (B) or after permeabilization (C). Numbers in quadrants represent the percentage of single- or double-stained cells with respect to the total number of viable cells analyzed.